This latest cash injection values the microblogging site at $8bn, up from a previous valuation of $3.7bn after the last funding round in December.
Twitter has not disclosed the terms of the deal: the $400m figure has been extracted from recent regulatory filings by the firm. But the size of the investment and the new valuation are in line with Twitter's growth.
Last year, users tweeted 65 million times a day. In 2011, that figure has more than tripled with 200 million tweets sent every day. And that's not all. As Twitter states in its blog: "One year ago, there were approximately 150,000 registered Twitter apps. Now, there are more than one million that connect to Twitter. And our team has grown from 250 people to more than 600 in the past 12 months."
DST is a good fit for the social platform. The VC has also taken stakes in a number of other firms in the space, including Facebook, Spotify, Zynga and Groupon. Some of Twitter's existing investors also took part in this latest round.
Twitter says: "We've come very far in a short time. Now we have an opportunity to expand Twitter's reach with a significant round of funding led by the venture firm DST Global, with the participation of several of our existing investors."